ABSTRACT
The TNA mRNA expression in 33 samples of cervical carcinoma and 28 samples of condyloma have been observed by using ~3H and Digoxigenin labeled TNF—? cDNA probes and in situ hybridization technique, we found that the results of in situ hybrdfzation with these two kinds of labeled probes were alike, the sensitivity with ~3H labeled probe was slightly higher than that with Digoxigenin labeled probe. Nevertheless, the nonisotope probe would be used more and more in future because of its safety, rapidity and convenience in work.
ABSTRACT
The development of transitional epithelium of ureter and urinary bladder in the rats from prenatal 15 days old to postnatal 4 weeks old was studied by histological and histochemical methods. During the development, RNA and glycogen contents are increased at first and then decreased. The activity of SDH,AcP and AlP increased gradually and ATPase reaction was negative. These parameters tended to be stable from the postnatal 3rd week, it meants that the epithelium tend to maturation. On the prenatal 15th day there were some significant differences between the epithelium of the two organs, however thereafter they gradually become identical and they showed the same type of epithelium, i.e. transitional epithelium at maturation. This means that they reached the same goal by different routes. In addition, on the prenatal 15th day the epithelium of urinary bladder stratified in most portions, some of the superficial cells degenerate. Hence it deduced that the epithelium of urinary bladder undergo primitive stratification firstly, nad then cell degeneration happens, and evolve into trasitional form ultimately. Meanwhile, We presume that in certain extent, the development of the epithelium of the two organs, including cell temporary degeneration, in addition to the action of embryonic induction, probably related to the content of urinary tract also.